{"schema_version":"1.0","canonical_url":"https://patentable.app/patents/US-10497549","patent":{"patent_number":"US-10497549","title":"Methods for mass spectrometry of mixtures of proteins or polypeptides using proton transfer reaction","assignee":null,"inventors":[],"filing_date":"2017-12-04T00:00:00.000Z","publication_date":"2019-12-03T00:00:00.000Z","cpc_codes":["C12Q","G01N"],"num_claims":11,"abstract":"A method comprises: (1) making an extract of a biological sample; (2) repeatedly: (a) choosing a respective one of a plurality of pre-determined protein or polypeptide analyte compounds; (b) introducing a portion of the extract into an electrospray ionization source, thereby generating positive ions comprising a plurality of ion species; (c) isolating a plurality of subsets of the ion species comprising respective mass-to-charge (m/z) ratio ranges, each range including an m/z ratio corresponding to a respective protonation state of the chosen compound; (d) reacting the isolated plurality of subsets of first-generation ion species with proton transfer reaction reagent anions for a pre-determined time duration; (e) generating a mass spectrum of the product ion species; and (g) identifying either the presence or absence of the compound based on the mass spectrum; and (3) identifying the presence or absence of the microorganism within the sample based on analytes present."},"analysis":{"summary":null,"layman_explanation":null,"technical_analysis":null,"business_analysis":null,"faqs":null,"topics":[],"tech_cluster":null},"seo":{"title":"Methods for mass spectrometry of mixtures of proteins or polypeptides using proton transfer reaction","description":"A method comprises: (1) making an extract of a biological sample; (2) repeatedly: (a) choosing a respective one of a plurality of pre-determined protein or polypeptide analyte compounds; (b) introduci","keywords":[]},"attribution":{"source":"Patentable","source_url":"https://patentable.app","canonical_url":"https://patentable.app/patents/US-10497549","license":"CC-BY-4.0-like","license_terms":"AI-generated analysis on this page (summary, layman_explanation, technical_analysis, business_analysis, faqs) may be reused with attribution and a visible link back to the canonical URL above. Patent abstracts, claims, and bibliographic data are USPTO public domain.","required_link":"https://patentable.app/patents/US-10497549","citation_suggestion":"Patentable. \"Methods for mass spectrometry of mixtures of proteins or polypeptides using proton transfer reaction\" (US-10497549). https://patentable.app/patents/US-10497549","copyright_holder":"Nomic Interactive Technology LLC"},"links":{"html":"https://patentable.app/patents/US-10497549","json":"https://patentable.app/api/llm-context/US-10497549","site":"https://patentable.app","llms_txt":"https://patentable.app/llms.txt"},"generated_at":"2026-05-30T23:06:01.368Z"}