{"schema_version":"1.0","canonical_url":"https://patentable.app/patents/US-10501814","patent":{"patent_number":"US-10501814","title":"Microarray based multiplex pathogen analysis and uses thereof","assignee":null,"inventors":[],"filing_date":"2018-10-11T00:00:00.000Z","publication_date":"2019-12-10T00:00:00.000Z","cpc_codes":["C12Q","C12Q","C12Q","C12Q","C12Q","C12Q","C12Q","C12Q","G16B","G16B","G16B"],"num_claims":10,"abstract":"Provided herein is a dual amplification method for detecting plant pathogens by analysis of pathogen DNA in an unpurified nucleic acid sample from the plant. Pathogen-specific primers are used to generate a first set of amplicons that are further amplified in a second amplification step using fluorescent tagged pathogen-specific primers. Fluorescent amplicons thus generated are hybridized with pathogen-specific nucleic acid probes that are immobilized on a solid support using bifunctional polymer linkers. The hybridized microarray is imaged to obtain fluorescent images of the amplicons and the nucleic acid probes, which are superimposed to detect the pathogen present in the plant. Also described herein is a method to simultaneously detect both plant DNA and pathogen DNA in a single assay."},"analysis":{"summary":null,"layman_explanation":null,"technical_analysis":null,"business_analysis":null,"faqs":null,"topics":[],"tech_cluster":null},"seo":{"title":"Microarray based multiplex pathogen analysis and uses thereof","description":"Provided herein is a dual amplification method for detecting plant pathogens by analysis of pathogen DNA in an unpurified nucleic acid sample from the plant. Pathogen-specific primers are used to gene","keywords":[]},"attribution":{"source":"Patentable","source_url":"https://patentable.app","canonical_url":"https://patentable.app/patents/US-10501814","license":"CC-BY-4.0-like","license_terms":"AI-generated analysis on this page (summary, layman_explanation, technical_analysis, business_analysis, faqs) may be reused with attribution and a visible link back to the canonical URL above. Patent abstracts, claims, and bibliographic data are USPTO public domain.","required_link":"https://patentable.app/patents/US-10501814","citation_suggestion":"Patentable. \"Microarray based multiplex pathogen analysis and uses thereof\" (US-10501814). https://patentable.app/patents/US-10501814","copyright_holder":"Nomic Interactive Technology LLC"},"links":{"html":"https://patentable.app/patents/US-10501814","json":"https://patentable.app/api/llm-context/US-10501814","site":"https://patentable.app","llms_txt":"https://patentable.app/llms.txt"},"generated_at":"2026-05-31T03:13:35.238Z"}