{"schema_version":"1.0","canonical_url":"https://patentable.app/patents/US-10508311","patent":{"patent_number":"US-10508311","title":"Single molecule-overlapping read analysis for minor variant mutation detection in pathogen samples","assignee":null,"inventors":[],"filing_date":"2014-08-26T00:00:00.000Z","publication_date":"2019-12-17T00:00:00.000Z","cpc_codes":["C12Q","C12Q","G16B","G16B","C12Q","C12Q"],"num_claims":16,"abstract":"The present invention provides a method of detecting a heteroresistant population of a pathogen in a sample, the method comprising: a) providing a sample comprising a population of a pathogen; b) extracting nucleic acids from the sample; c) amplifying a target locus of the genome of the pathogen in the extracted nucleic acids, wherein the target locus comprises at least one minor variant associated with drug resistance in the pathogen; d) consecutively sequencing both overlapping nucleic acid strands from a single DNA molecule amplified from the target locus on a Next Generation Sequencing (NGS) platform; e) applying an alignment algorithm to sequencing data from the overlapping nucleic acid strands; and f) performing an analysis of the aligned sequencing data to detect the at least one minor variant and heteroresistant population of the pathogen."},"analysis":{"summary":null,"layman_explanation":null,"technical_analysis":null,"business_analysis":null,"faqs":null,"topics":[],"tech_cluster":null},"seo":{"title":"Single molecule-overlapping read analysis for minor variant mutation detection in pathogen samples","description":"The present invention provides a method of detecting a heteroresistant population of a pathogen in a sample, the method comprising: a) providing a sample comprising a population of a pathogen; b) extr","keywords":[]},"attribution":{"source":"Patentable","source_url":"https://patentable.app","canonical_url":"https://patentable.app/patents/US-10508311","license":"CC-BY-4.0-like","license_terms":"AI-generated analysis on this page (summary, layman_explanation, technical_analysis, business_analysis, faqs) may be reused with attribution and a visible link back to the canonical URL above. Patent abstracts, claims, and bibliographic data are USPTO public domain.","required_link":"https://patentable.app/patents/US-10508311","citation_suggestion":"Patentable. \"Single molecule-overlapping read analysis for minor variant mutation detection in pathogen samples\" (US-10508311). https://patentable.app/patents/US-10508311","copyright_holder":"Nomic Interactive Technology LLC"},"links":{"html":"https://patentable.app/patents/US-10508311","json":"https://patentable.app/api/llm-context/US-10508311","site":"https://patentable.app","llms_txt":"https://patentable.app/llms.txt"},"generated_at":"2026-05-30T17:36:43.326Z"}