{"schema_version":"1.0","canonical_url":"https://patentable.app/patents/US-10544453","patent":{"patent_number":"US-10544453","title":"Method for detecting nucleic acid amplification product in real time","assignee":null,"inventors":[],"filing_date":"2014-07-23T00:00:00.000Z","publication_date":"2020-01-28T00:00:00.000Z","cpc_codes":["C12Q","C12Q","G01N"],"num_claims":3,"abstract":"There is provided an apparatus for detecting a nucleic acid amplification product in real time, which is capable of effectively excluding or reducing apparatus error factors without using a second fluorescence signal used for correction. A plurality of wells 7A are given with temperature cycles and fluorescence strength from a nucleic acid amplification product is detected in real time in each well 7A. A fluorescence measurement value [DNA]raw obtained from the well 7A and a fluorescence measurement value [DNA]bg obtained from a connection wall near the well 7A are detected, and the fluorescence measurement value [DNA]bg is subtracted from the fluorescence measurement value [DNA]raw to determine fluorescence strength [DNA]real of the well 7A."},"analysis":{"summary":null,"layman_explanation":null,"technical_analysis":null,"business_analysis":null,"faqs":null,"topics":[],"tech_cluster":null},"seo":{"title":"Method for detecting nucleic acid amplification product in real time","description":"There is provided an apparatus for detecting a nucleic acid amplification product in real time, which is capable of effectively excluding or reducing apparatus error factors without using a second flu","keywords":[]},"attribution":{"source":"Patentable","source_url":"https://patentable.app","canonical_url":"https://patentable.app/patents/US-10544453","license":"CC-BY-4.0-like","license_terms":"AI-generated analysis on this page (summary, layman_explanation, technical_analysis, business_analysis, faqs) may be reused with attribution and a visible link back to the canonical URL above. Patent abstracts, claims, and bibliographic data are USPTO public domain.","required_link":"https://patentable.app/patents/US-10544453","citation_suggestion":"Patentable. \"Method for detecting nucleic acid amplification product in real time\" (US-10544453). https://patentable.app/patents/US-10544453","copyright_holder":"Nomic Interactive Technology LLC"},"links":{"html":"https://patentable.app/patents/US-10544453","json":"https://patentable.app/api/llm-context/US-10544453","site":"https://patentable.app","llms_txt":"https://patentable.app/llms.txt"},"generated_at":"2026-05-31T04:21:20.979Z"}