{"schema_version":"1.0","canonical_url":"https://patentable.app/patents/US-11276478","patent":{"patent_number":"US-11276478","title":"Methods and systems for quantifying gene expression in synthetic gene circuits and for tuning synthetic gene circuits","assignee":null,"inventors":[],"filing_date":"2019-01-15T00:00:00.000Z","publication_date":"2022-03-15T00:00:00.000Z","cpc_codes":["G16B","G16B"],"num_claims":20,"abstract":"A method of tuning the performance of a synthetic gene circuit comprising a plurality of genes is disclosed. The method includes quantifying, for each gene, a relative gene expression metric. The metric is quantified by determining a number of nucleotides in a 5′ adjacent transcriptional region (ATR) of the gene, a number of nucleotides in a 3′ ATR, a minimum free energy of a mRNA secondary structure around a ribosome binding site of the gene, a total folding energy for the 5′ ATR, and a folding energy for the first 100 nucleotides of the 3′ ATR, and then summing sequence-dependent energy changes, the terms including scaling coefficients and an average energy cost for synthesizing a nucleotide. The method further includes simulating, through application of the relative gene expression metrics of the plurality of genes to a deterministic model, the performance of an intended function of the synthetic gene circuit."},"analysis":{"summary":null,"layman_explanation":null,"technical_analysis":null,"business_analysis":null,"faqs":null,"topics":[],"tech_cluster":null},"seo":{"title":"Methods and systems for quantifying gene expression in synthetic gene circuits and for tuning synthetic gene circuits","description":"A method of tuning the performance of a synthetic gene circuit comprising a plurality of genes is disclosed. The method includes quantifying, for each gene, a relative gene expression metric. The metr","keywords":[]},"attribution":{"source":"Patentable","source_url":"https://patentable.app","canonical_url":"https://patentable.app/patents/US-11276478","license":"CC-BY-4.0-like","license_terms":"AI-generated analysis on this page (summary, layman_explanation, technical_analysis, business_analysis, faqs) may be reused with attribution and a visible link back to the canonical URL above. Patent abstracts, claims, and bibliographic data are USPTO public domain.","required_link":"https://patentable.app/patents/US-11276478","citation_suggestion":"Patentable. \"Methods and systems for quantifying gene expression in synthetic gene circuits and for tuning synthetic gene circuits\" (US-11276478). https://patentable.app/patents/US-11276478","copyright_holder":"Nomic Interactive Technology LLC"},"links":{"html":"https://patentable.app/patents/US-11276478","json":"https://patentable.app/api/llm-context/US-11276478","site":"https://patentable.app","llms_txt":"https://patentable.app/llms.txt"},"generated_at":"2026-05-30T20:06:20.031Z"}