{"schema_version":"1.0","canonical_url":"https://patentable.app/patents/US-11286514","patent":{"patent_number":"US-11286514","title":"T5 exonuclease-based method to identify DNA topoisomerase inhibitors","assignee":null,"inventors":[],"filing_date":"2020-10-16T00:00:00.000Z","publication_date":"2022-03-29T00:00:00.000Z","cpc_codes":["C12Q","C12Q","G01N","G01N","G01N","G01N"],"num_claims":12,"abstract":"The present invention provides assays and methods for studying DNA topology and topoisomerases. The assays and methods utilize a circular plasmid DNA comprising one or more hairpin structures and the ability of T5 exonuclease (T5E) to digest the circular plasmid DNA in a specific configuration. The assays and methods can be used as a high throughput screening for inhibitors of, for example, DNA gyrases and DNA topoisomerases I for anticancer drug and antibiotics discovery."},"analysis":{"summary":null,"layman_explanation":null,"technical_analysis":null,"business_analysis":null,"faqs":null,"topics":[],"tech_cluster":null},"seo":{"title":"T5 exonuclease-based method to identify DNA topoisomerase inhibitors","description":"The present invention provides assays and methods for studying DNA topology and topoisomerases. The assays and methods utilize a circular plasmid DNA comprising one or more hairpin structures and the ","keywords":[]},"attribution":{"source":"Patentable","source_url":"https://patentable.app","canonical_url":"https://patentable.app/patents/US-11286514","license":"CC-BY-4.0-like","license_terms":"AI-generated analysis on this page (summary, layman_explanation, technical_analysis, business_analysis, faqs) may be reused with attribution and a visible link back to the canonical URL above. Patent abstracts, claims, and bibliographic data are USPTO public domain.","required_link":"https://patentable.app/patents/US-11286514","citation_suggestion":"Patentable. \"T5 exonuclease-based method to identify DNA topoisomerase inhibitors\" (US-11286514). https://patentable.app/patents/US-11286514","copyright_holder":"Nomic Interactive Technology LLC"},"links":{"html":"https://patentable.app/patents/US-11286514","json":"https://patentable.app/api/llm-context/US-11286514","site":"https://patentable.app","llms_txt":"https://patentable.app/llms.txt"},"generated_at":"2026-05-30T13:26:36.436Z"}