{"schema_version":"1.0","canonical_url":"https://patentable.app/patents/US-11512342","patent":{"patent_number":"US-11512342","title":"Compositions and methods for detecting pyrophosphate products of enzyme reactions using pyridylazoaniline dyes","assignee":null,"inventors":[],"filing_date":"2022-05-04T00:00:00.000Z","publication_date":"2022-11-29T00:00:00.000Z","cpc_codes":["C12Q","C12Q","C12Q","C12Q","G01N"],"num_claims":29,"abstract":"Provided herein is a composition comprising an enzyme that releases pyrophosphate from a substrate and a dye of Formula 1. A method for detecting pyrophosphate is also provided. A kit comprising a polymerase that releases pyrophosphate by hydrolysis of nucleoside triphosphates during nucleic acid replication, a divalent manganese salt, and the dye are also provided. The present composition, method and kits provide a way to detect and/or quantify substrates or products of enzyme reacted substrates associated with the release pyrophosphate (e.g., nucleic acid amplification reactions and other reactions that hydrolyze ATP) via a distinct color change without substantially affecting the sensitivity and/or specificity of the reaction."},"analysis":{"summary":null,"layman_explanation":null,"technical_analysis":null,"business_analysis":null,"faqs":null,"topics":[],"tech_cluster":null},"seo":{"title":"Compositions and methods for detecting pyrophosphate products of enzyme reactions using pyridylazoaniline dyes","description":"Provided herein is a composition comprising an enzyme that releases pyrophosphate from a substrate and a dye of Formula 1. A method for detecting pyrophosphate is also provided. A kit comprising a pol","keywords":[]},"attribution":{"source":"Patentable","source_url":"https://patentable.app","canonical_url":"https://patentable.app/patents/US-11512342","license":"CC-BY-4.0-like","license_terms":"AI-generated analysis on this page (summary, layman_explanation, technical_analysis, business_analysis, faqs) may be reused with attribution and a visible link back to the canonical URL above. Patent abstracts, claims, and bibliographic data are USPTO public domain.","required_link":"https://patentable.app/patents/US-11512342","citation_suggestion":"Patentable. \"Compositions and methods for detecting pyrophosphate products of enzyme reactions using pyridylazoaniline dyes\" (US-11512342). https://patentable.app/patents/US-11512342","copyright_holder":"Nomic Interactive Technology LLC"},"links":{"html":"https://patentable.app/patents/US-11512342","json":"https://patentable.app/api/llm-context/US-11512342","site":"https://patentable.app","llms_txt":"https://patentable.app/llms.txt"},"generated_at":"2026-05-30T14:59:22.505Z"}