{"schema_version":"1.0","canonical_url":"https://patentable.app/patents/US-11525814","patent":{"patent_number":"US-11525814","title":"Method for screening of tobacco nicotine converters","assignee":null,"inventors":[],"filing_date":"2020-12-29T00:00:00.000Z","publication_date":"2022-12-13T00:00:00.000Z","cpc_codes":["G01N","G01N","G01N","G01N","G01N","G01N","G01N","G01N","G01N"],"num_claims":16,"abstract":"A method for identifying converters from tobacco seedling population. The method includes: 1) sowing and cultivating tobacco seeds to be identified for 45-55 days; sampling a plurality of leaf disks from each of 45-55 days old seedlings; 2) incubating the plurality of leaf disks of each seedling in a sealed container at 37° C. for 10-12 hours, thereby obtaining a plurality of incubated tobacco leaves of each seedling; 3) immersing the plurality of incubated tobacco leaves of each seedling in an extractant, extracting alkaloids and obtaining an extract of each seedling; 4) analyzing the amounts of nicotine and nornicotine in the alkaloids extract of each seedling; and 5) automatically recognizing peaks of the alkaloids extract of each seedling, and calculating the percent nicotine conversion (PNC) and the pseudo percent nicotine conversion (PPNC)."},"analysis":{"summary":null,"layman_explanation":null,"technical_analysis":null,"business_analysis":null,"faqs":null,"topics":[],"tech_cluster":null},"seo":{"title":"Method for screening of tobacco nicotine converters","description":"A method for identifying converters from tobacco seedling population. The method includes: 1) sowing and cultivating tobacco seeds to be identified for 45-55 days; sampling a plurality of leaf disks f","keywords":[]},"attribution":{"source":"Patentable","source_url":"https://patentable.app","canonical_url":"https://patentable.app/patents/US-11525814","license":"CC-BY-4.0-like","license_terms":"AI-generated analysis on this page (summary, layman_explanation, technical_analysis, business_analysis, faqs) may be reused with attribution and a visible link back to the canonical URL above. Patent abstracts, claims, and bibliographic data are USPTO public domain.","required_link":"https://patentable.app/patents/US-11525814","citation_suggestion":"Patentable. \"Method for screening of tobacco nicotine converters\" (US-11525814). https://patentable.app/patents/US-11525814","copyright_holder":"Nomic Interactive Technology LLC"},"links":{"html":"https://patentable.app/patents/US-11525814","json":"https://patentable.app/api/llm-context/US-11525814","site":"https://patentable.app","llms_txt":"https://patentable.app/llms.txt"},"generated_at":"2026-05-31T11:54:15.260Z"}