{"schema_version":"1.0","canonical_url":"https://patentable.app/patents/US-11960102","patent":{"patent_number":"US-11960102","title":"Pinpointing single molecule positions with in situ point spread function retrieval for three dimensional nanoscopy of whole cells and tissues","assignee":null,"inventors":[],"filing_date":"2020-08-06T00:00:00.000Z","publication_date":"2024-04-16T00:00:00.000Z","cpc_codes":["G01N","G06T"],"num_claims":20,"abstract":"A method of in situ point spread function (PSF) retrieval is disclosed which includes encoding 3D location of molecules into PSFs, receiving molecule-generated images containing PSFs, segmenting the images into sub-PSFs, initializing template PSFs from a pupil function, determining a maximum normalized cross correlation (NCC) coefficient (NCCmax) between the sub- and template PSFs, associating each of the sub-PSFs with a template PSF based on the NCCmax and storing the sub-PSFs in associated bins, aligning and averaging the binned sub-PSFs, applying a phase retrieval algorithm to the averaged sub-PSFs to update the pupil function, regenerating the template PSFs, repeating until a difference between a new and a prior generation pupil function is below a predetermined threshold, generating in situ PSFs from the last pupil function, and applying a maximum likelihood estimation algorithm based on the in situ PSFs and the sub-PSFs to thereby generate lateral and axial locations of molecules."},"analysis":{"summary":null,"layman_explanation":null,"technical_analysis":null,"business_analysis":null,"faqs":null,"topics":[],"tech_cluster":null},"seo":{"title":"Pinpointing single molecule positions with in situ point spread function retrieval for three dimensional nanoscopy of whole cells and tissues","description":"A method of in situ point spread function (PSF) retrieval is disclosed which includes encoding 3D location of molecules into PSFs, receiving molecule-generated images containing PSFs, segmenting the i","keywords":[]},"attribution":{"source":"Patentable","source_url":"https://patentable.app","canonical_url":"https://patentable.app/patents/US-11960102","license":"CC-BY-4.0-like","license_terms":"AI-generated analysis on this page (summary, layman_explanation, technical_analysis, business_analysis, faqs) may be reused with attribution and a visible link back to the canonical URL above. Patent abstracts, claims, and bibliographic data are USPTO public domain.","required_link":"https://patentable.app/patents/US-11960102","citation_suggestion":"Patentable. \"Pinpointing single molecule positions with in situ point spread function retrieval for three dimensional nanoscopy of whole cells and tissues\" (US-11960102). https://patentable.app/patents/US-11960102","copyright_holder":"Nomic Interactive Technology LLC"},"links":{"html":"https://patentable.app/patents/US-11960102","json":"https://patentable.app/api/llm-context/US-11960102","site":"https://patentable.app","llms_txt":"https://patentable.app/llms.txt"},"generated_at":"2026-05-31T00:15:27.866Z"}