{"schema_version":"1.0","canonical_url":"https://patentable.app/patents/US-11970726","patent":{"patent_number":"US-11970726","title":"Method for quantifying the cultivability of individual bacterial cells using culture independent parameters","assignee":null,"inventors":[],"filing_date":"2018-06-28T00:00:00.000Z","publication_date":"2024-04-30T00:00:00.000Z","cpc_codes":["C12Q","G01N","G06F"],"num_claims":13,"abstract":"The invention relates to the field of microbiology and detecting micro-organism. Provided is a method for the quantitative analysis of microbial cells in a liquid sample, comprising the steps of (i) contacting the sample with a nucleic acid binding fluorescent dye to stain for nucleic acids in said microbial cells; (ii) subjecting the labelled cells to a fluorescence microscopy imaging method and determining, based on the nucleic acid staining, for a plurality of single cells at least a first parameter reflecting the nucleic acid content of said cell and a second parameter reflecting the extent of stretching of said cell; (iii) classifying based on said at least first and second parameter said cell into one of the following categories: (A) Viable cultivable cells with a nucleic acid content value between >=0.1 and <1.0 and extent of stretching of the cell value between >=1.0 and 10 μιηm2, (B) Viable non-cultivable cells with a nucleic acid content value of >−0.2 and extent of stretching of the cell value of >=0.5 μιηm2 minus (A), (C) Most likely dead cells with a nucleic acid content of <1.0 and extent of stretching of the cell value of <0.50 μιηm2; and (iv) calculating the ratio of the amount of (A) viable cultivable cells, (B) viable non-cultivable cells and (C) most likely dead cells in a microbial sample."},"analysis":{"summary":null,"layman_explanation":null,"technical_analysis":null,"business_analysis":null,"faqs":null,"topics":[],"tech_cluster":null},"seo":{"title":"Method for quantifying the cultivability of individual bacterial cells using culture independent parameters","description":"The invention relates to the field of microbiology and detecting micro-organism. Provided is a method for the quantitative analysis of microbial cells in a liquid sample, comprising the steps of (i) c","keywords":[]},"attribution":{"source":"Patentable","source_url":"https://patentable.app","canonical_url":"https://patentable.app/patents/US-11970726","license":"CC-BY-4.0-like","license_terms":"AI-generated analysis on this page (summary, layman_explanation, technical_analysis, business_analysis, faqs) may be reused with attribution and a visible link back to the canonical URL above. Patent abstracts, claims, and bibliographic data are USPTO public domain.","required_link":"https://patentable.app/patents/US-11970726","citation_suggestion":"Patentable. \"Method for quantifying the cultivability of individual bacterial cells using culture independent parameters\" (US-11970726). https://patentable.app/patents/US-11970726","copyright_holder":"Nomic Interactive Technology LLC"},"links":{"html":"https://patentable.app/patents/US-11970726","json":"https://patentable.app/api/llm-context/US-11970726","site":"https://patentable.app","llms_txt":"https://patentable.app/llms.txt"},"generated_at":"2026-05-31T10:41:00.805Z"}