{"schema_version":"1.0","canonical_url":"https://patentable.app/patents/US-8507295","patent":{"patent_number":"US-8507295","title":"Methods of quantification for lateral flow devices","assignee":null,"inventors":[],"filing_date":"2008-11-10T00:00:00.000Z","publication_date":"2013-08-13T00:00:00.000Z","cpc_codes":["G01N","G01N"],"num_claims":11,"abstract":"The invention concerns methods of quantification of an analyte, A, in a test sample by means of a single immunochromatographic device, such as a lateral flow device (LFD). One method comprises the steps of: a) mixing a determined amount of said test sample with a determined amount of a quantification agent, QA1, said QA1 being capable of binding specifically and simultaneously both to an immobilized binding partner and to A, thus obtaining a mixture wherein the amount of A in said test sample is reflected by the ratio of the concentration of A-QA1 complex formed to the concentration of free QA1 in said mixture; b) applying a determined volume of said mixture to said device equipped with a test band in which said binding partner of the QA1 is immobilized, so that A-QA1 complex and free QA1 are immobilized at said test band in a ratio that relates to their concentration ratio in the mixture applied, said volume of mixture being determined to provide a total amount of A-QA1 complex and free QA1 capable of sufficiently saturating the immobilized binding partner in said test band; c) measuring the amount of either said A-QA1 complex or said free QA1 immobilized at the test band by means of an appropriately chosen detection procedure; d) comparing the result obtained from the test sample with the results obtained from calibration samples containing known or allocated amounts of A and thus determining the amount of A in said test sample. By including the two procedural stages a) and b) this greatly diminish or eliminate the sources of run-to-run variation."},"analysis":{"summary":null,"layman_explanation":null,"technical_analysis":null,"business_analysis":null,"faqs":null,"topics":[],"tech_cluster":null},"seo":{"title":"Methods of quantification for lateral flow devices","description":"The invention concerns methods of quantification of an analyte, A, in a test sample by means of a single immunochromatographic device, such as a lateral flow device (LFD). One method comprises the ste","keywords":[]},"attribution":{"source":"Patentable","source_url":"https://patentable.app","canonical_url":"https://patentable.app/patents/US-8507295","license":"CC-BY-4.0-like","license_terms":"AI-generated analysis on this page (summary, layman_explanation, technical_analysis, business_analysis, faqs) may be reused with attribution and a visible link back to the canonical URL above. Patent abstracts, claims, and bibliographic data are USPTO public domain.","required_link":"https://patentable.app/patents/US-8507295","citation_suggestion":"Patentable. \"Methods of quantification for lateral flow devices\" (US-8507295). https://patentable.app/patents/US-8507295","copyright_holder":"Nomic Interactive Technology LLC"},"links":{"html":"https://patentable.app/patents/US-8507295","json":"https://patentable.app/api/llm-context/US-8507295","site":"https://patentable.app","llms_txt":"https://patentable.app/llms.txt"},"generated_at":"2026-05-30T21:45:15.771Z"}