{"schema_version":"1.0","canonical_url":"https://patentable.app/patents/US-8510054","patent":{"patent_number":"US-8510054","title":"Intracellular metabolic flux analysis method using substrate labeled with isotope","assignee":null,"inventors":[],"filing_date":"2005-02-03T00:00:00.000Z","publication_date":"2013-08-13T00:00:00.000Z","cpc_codes":["G01N","C12Q","G16B","G16B"],"num_claims":11,"abstract":"A method for intracellular metabolic flux analysis comprising (a) culturing cells in a medium not containing any isotope-labeled substrate to a target phase of the metabolic flux analysis, (b) adding an isotope-labeled substrate to the medium, and continuing culture and collecting samples from the medium in time course, (c) measuring isotope distribution in an intracellular metabolite contained in the samples collected in time course, (d) performing a regression analysis for measured data and calculating an isotope distribution ratio in a steady state, and (e) analyzing a metabolic flux of the cultured cells by using the calculated isotope distribution ratio."},"analysis":{"summary":null,"layman_explanation":null,"technical_analysis":null,"business_analysis":null,"faqs":null,"topics":[],"tech_cluster":null},"seo":{"title":"Intracellular metabolic flux analysis method using substrate labeled with isotope","description":"A method for intracellular metabolic flux analysis comprising (a) culturing cells in a medium not containing any isotope-labeled substrate to a target phase of the metabolic flux analysis, (b) adding ","keywords":[]},"attribution":{"source":"Patentable","source_url":"https://patentable.app","canonical_url":"https://patentable.app/patents/US-8510054","license":"CC-BY-4.0-like","license_terms":"AI-generated analysis on this page (summary, layman_explanation, technical_analysis, business_analysis, faqs) may be reused with attribution and a visible link back to the canonical URL above. Patent abstracts, claims, and bibliographic data are USPTO public domain.","required_link":"https://patentable.app/patents/US-8510054","citation_suggestion":"Patentable. \"Intracellular metabolic flux analysis method using substrate labeled with isotope\" (US-8510054). https://patentable.app/patents/US-8510054","copyright_holder":"Nomic Interactive Technology LLC"},"links":{"html":"https://patentable.app/patents/US-8510054","json":"https://patentable.app/api/llm-context/US-8510054","site":"https://patentable.app","llms_txt":"https://patentable.app/llms.txt"},"generated_at":"2026-05-30T16:19:27.488Z"}