{"schema_version":"1.0","canonical_url":"https://patentable.app/patents/US-9603949","patent":{"patent_number":"US-9603949","title":"Oligonucleotide-based probes for detection of bacterial nucleases","assignee":null,"inventors":[],"filing_date":"2012-08-30T00:00:00.000Z","publication_date":"2017-03-28T00:00:00.000Z","cpc_codes":["C12Q","C12Q","C12Q","C12Q","G01N","G01N","G01N"],"num_claims":21,"abstract":"The present invention relates to a rapid detection of microbial-associated nuclease activity with chemically modified nuclease (e.g., ribonuclease) substrates, and probes and compositions useful in detection assays. Accordingly, in certain embodiments, the present invention provides a probe for detecting a microbial endonuclease comprising a substrate oligonucleotide of 2-30 nucleotides in length, a fluorescence-reporter group operably linked to the oligonucleotide, and a fluorescence-quencher group operably linked to the oligonucleotide. The fluorescence-reporter group and the fluorescence-quencher group are separated by at least one RNAse-cleavable residue, e.g., RNA base."},"analysis":{"summary":null,"layman_explanation":null,"technical_analysis":null,"business_analysis":null,"faqs":null,"topics":[],"tech_cluster":null},"seo":{"title":"Oligonucleotide-based probes for detection of bacterial nucleases","description":"The present invention relates to a rapid detection of microbial-associated nuclease activity with chemically modified nuclease (e.g., ribonuclease) substrates, and probes and compositions useful in de","keywords":[]},"attribution":{"source":"Patentable","source_url":"https://patentable.app","canonical_url":"https://patentable.app/patents/US-9603949","license":"CC-BY-4.0-like","license_terms":"AI-generated analysis on this page (summary, layman_explanation, technical_analysis, business_analysis, faqs) may be reused with attribution and a visible link back to the canonical URL above. Patent abstracts, claims, and bibliographic data are USPTO public domain.","required_link":"https://patentable.app/patents/US-9603949","citation_suggestion":"Patentable. \"Oligonucleotide-based probes for detection of bacterial nucleases\" (US-9603949). https://patentable.app/patents/US-9603949","copyright_holder":"Nomic Interactive Technology LLC"},"links":{"html":"https://patentable.app/patents/US-9603949","json":"https://patentable.app/api/llm-context/US-9603949","site":"https://patentable.app","llms_txt":"https://patentable.app/llms.txt"},"generated_at":"2026-06-06T06:37:06.881Z"}