{"schema_version":"1.0","canonical_url":"https://patentable.app/patents/US-9625468","patent":{"patent_number":"US-9625468","title":"Method of isolating and analyzing oligosaccharides in glycoproteins","assignee":null,"inventors":[],"filing_date":"2015-11-19T00:00:00.000Z","publication_date":"2017-04-18T00:00:00.000Z","cpc_codes":["G01N","G01N"],"num_claims":15,"abstract":"A method for isolating derivatives of general O-linked amino acids including derivatives of O-tyrosine linked kappa casein di-O-sulfo tetrasaccharide and of O-di-phospho serine linked bovine submaxillary mucin disaccharide is provided. These derivatives are isolated and analyzed by further enzymatically separating the N-linked oligosaccharides from the O-linked oligosaccharides and then cleave O-linked oligosaccharides by decreasing the pH levels such that de-amidations are allowed. This de-amidation results in individual O-linked glycan amino acid components removed from the original glycoprotein. The O-linked glycan amino acid components are thus isolated from the glycoprotein, and can be individually analyzed."},"analysis":{"summary":null,"layman_explanation":null,"technical_analysis":null,"business_analysis":null,"faqs":null,"topics":[],"tech_cluster":null},"seo":{"title":"Method of isolating and analyzing oligosaccharides in glycoproteins","description":"A method for isolating derivatives of general O-linked amino acids including derivatives of O-tyrosine linked kappa casein di-O-sulfo tetrasaccharide and of O-di-phospho serine linked bovine submaxill","keywords":[]},"attribution":{"source":"Patentable","source_url":"https://patentable.app","canonical_url":"https://patentable.app/patents/US-9625468","license":"CC-BY-4.0-like","license_terms":"AI-generated analysis on this page (summary, layman_explanation, technical_analysis, business_analysis, faqs) may be reused with attribution and a visible link back to the canonical URL above. Patent abstracts, claims, and bibliographic data are USPTO public domain.","required_link":"https://patentable.app/patents/US-9625468","citation_suggestion":"Patentable. \"Method of isolating and analyzing oligosaccharides in glycoproteins\" (US-9625468). https://patentable.app/patents/US-9625468","copyright_holder":"Nomic Interactive Technology LLC"},"links":{"html":"https://patentable.app/patents/US-9625468","json":"https://patentable.app/api/llm-context/US-9625468","site":"https://patentable.app","llms_txt":"https://patentable.app/llms.txt"},"generated_at":"2026-06-06T10:18:01.831Z"}