{"schema_version":"1.0","canonical_url":"https://patentable.app/patents/US-9657333","patent":{"patent_number":"US-9657333","title":"Method for detecting target substance, assay kit, and detection apparatus","assignee":null,"inventors":[],"filing_date":"2013-02-19T00:00:00.000Z","publication_date":"2017-05-23T00:00:00.000Z","cpc_codes":["C12Q","C12Q","G01N","G01N"],"num_claims":10,"abstract":"The present invention provides a method for detecting a target substance, including the steps of: preparing a complex (hybrid) comprising an aptamer which binds to the target substance, a first nucleic acid fragment comprising a nucleotide sequence complementary to the aptamer and a photoisomerizable molecule bound to a portion of the complementary nucleotide sequence, wherein the aptamer and the first nucleic acid fragment bound with the photoisomerizable molecule form double-stranded nucleotides binding; subjecting the photoisomerizable molecule to a first photoisomerization treatment to destabilize the double-stranded nucleotides binding; forming a complex of the target substance and the aptamer so as to dissolve the destabilized double-stranded nucleotides binding; subjecting the photoisomerizable molecule to a second photoisomerization treatment to restabilize double-stranded nucleotides binding; and detecting dissolution of double-stranded nucleotides binding wherein the first nucleic acid fragment bound with the photoisomerizable molecule separates from the aptamer."},"analysis":{"summary":null,"layman_explanation":null,"technical_analysis":null,"business_analysis":null,"faqs":null,"topics":[],"tech_cluster":null},"seo":{"title":"Method for detecting target substance, assay kit, and detection apparatus","description":"The present invention provides a method for detecting a target substance, including the steps of: preparing a complex (hybrid) comprising an aptamer which binds to the target substance, a first nuclei","keywords":[]},"attribution":{"source":"Patentable","source_url":"https://patentable.app","canonical_url":"https://patentable.app/patents/US-9657333","license":"CC-BY-4.0-like","license_terms":"AI-generated analysis on this page (summary, layman_explanation, technical_analysis, business_analysis, faqs) may be reused with attribution and a visible link back to the canonical URL above. Patent abstracts, claims, and bibliographic data are USPTO public domain.","required_link":"https://patentable.app/patents/US-9657333","citation_suggestion":"Patentable. \"Method for detecting target substance, assay kit, and detection apparatus\" (US-9657333). https://patentable.app/patents/US-9657333","copyright_holder":"Nomic Interactive Technology LLC"},"links":{"html":"https://patentable.app/patents/US-9657333","json":"https://patentable.app/api/llm-context/US-9657333","site":"https://patentable.app","llms_txt":"https://patentable.app/llms.txt"},"generated_at":"2026-06-06T09:00:33.443Z"}