{"schema_version":"1.0","canonical_url":"https://patentable.app/patents/US-9777042","patent":{"patent_number":"US-9777042","title":"Method of purifying HIV/SIV Nef from exosomal fusion proteins","assignee":null,"inventors":[],"filing_date":"2011-12-15T00:00:00.000Z","publication_date":"2017-10-03T00:00:00.000Z","cpc_codes":["G01N","G01N"],"num_claims":11,"abstract":"The present application relates to methods of producing exosomes. The application also provides a method for preparing a protein composition comprising culturing an exosome-producing cell expressing a Nef-fusion protein comprising a Nef-derived peptide fused to a protein of interest; isolating exosomes from the exosome-producing cell culture; and purifying the protein of interest from the isolated exosomes. The application further discloses compositions that comprise exosomes containing the Nef-fusion protein, as well as methods of using the Nef-fusion protein and exosomes containing the Nef-fusion protein."},"analysis":{"summary":null,"layman_explanation":null,"technical_analysis":null,"business_analysis":null,"faqs":null,"topics":[],"tech_cluster":null},"seo":{"title":"Method of purifying HIV/SIV Nef from exosomal fusion proteins","description":"The present application relates to methods of producing exosomes. The application also provides a method for preparing a protein composition comprising culturing an exosome-producing cell expressing a","keywords":[]},"attribution":{"source":"Patentable","source_url":"https://patentable.app","canonical_url":"https://patentable.app/patents/US-9777042","license":"CC-BY-4.0-like","license_terms":"AI-generated analysis on this page (summary, layman_explanation, technical_analysis, business_analysis, faqs) may be reused with attribution and a visible link back to the canonical URL above. Patent abstracts, claims, and bibliographic data are USPTO public domain.","required_link":"https://patentable.app/patents/US-9777042","citation_suggestion":"Patentable. \"Method of purifying HIV/SIV Nef from exosomal fusion proteins\" (US-9777042). https://patentable.app/patents/US-9777042","copyright_holder":"Nomic Interactive Technology LLC"},"links":{"html":"https://patentable.app/patents/US-9777042","json":"https://patentable.app/api/llm-context/US-9777042","site":"https://patentable.app","llms_txt":"https://patentable.app/llms.txt"},"generated_at":"2026-06-06T11:41:23.113Z"}