{"schema_version":"1.0","canonical_url":"https://patentable.app/patents/US-9803181","patent":{"patent_number":"US-9803181","title":"Hybrid alpha-amylases","assignee":null,"inventors":[],"filing_date":"2014-07-25T00:00:00.000Z","publication_date":"2017-10-31T00:00:00.000Z","cpc_codes":["G16B","G16B","G16B"],"num_claims":5,"abstract":"Hybrid alpha-amylases are provided that share a conserved 3D structure in whole or in part with a wild-type Termamyl-like α-amylase, e.g., a Bacillus amylase. In the hybrid, an N-terminal portion of a Termamyl-like α-amylase is replaced with sequences from an archae α-amylase. The sequence similarity between the two amylase sequences may be less than 60%. Conserving the wild-type 3D structure in the hybrid facilitates obtaining enzymatically active amylases. In one embodiment, one or both amylase sequences contribute residues to the B domain, resulting in particularly advantageous properties. For instance, replacement of the Ca2+ binding site in the B domain of the Termamyl-like α-amylase with a B domain sequence of an archae α-amylase that does not bind Ca2+ can produce a hybrid that is fully active in the absence of Ca2+."},"analysis":{"summary":null,"layman_explanation":null,"technical_analysis":null,"business_analysis":null,"faqs":null,"topics":[],"tech_cluster":null},"seo":{"title":"Hybrid alpha-amylases","description":"Hybrid alpha-amylases are provided that share a conserved 3D structure in whole or in part with a wild-type Termamyl-like α-amylase, e.g., a Bacillus amylase. In the hybrid, an N-terminal portion of a","keywords":[]},"attribution":{"source":"Patentable","source_url":"https://patentable.app","canonical_url":"https://patentable.app/patents/US-9803181","license":"CC-BY-4.0-like","license_terms":"AI-generated analysis on this page (summary, layman_explanation, technical_analysis, business_analysis, faqs) may be reused with attribution and a visible link back to the canonical URL above. Patent abstracts, claims, and bibliographic data are USPTO public domain.","required_link":"https://patentable.app/patents/US-9803181","citation_suggestion":"Patentable. \"Hybrid alpha-amylases\" (US-9803181). https://patentable.app/patents/US-9803181","copyright_holder":"Nomic Interactive Technology LLC"},"links":{"html":"https://patentable.app/patents/US-9803181","json":"https://patentable.app/api/llm-context/US-9803181","site":"https://patentable.app","llms_txt":"https://patentable.app/llms.txt"},"generated_at":"2026-06-06T07:16:57.491Z"}