{"schema_version":"1.0","canonical_url":"https://patentable.app/patents/US-9850382","patent":{"patent_number":"US-9850382","title":"Unsymmetrical cyanine dyes and their application","assignee":null,"inventors":[],"filing_date":"2016-12-05T00:00:00.000Z","publication_date":"2017-12-26T00:00:00.000Z","cpc_codes":["C12Q","G01N"],"num_claims":13,"abstract":"The invention describes the preparation and use of fluorescent stains for nucleic acids derived from unsymmetrical cyanine dyes. The dyes of the invention possess superior fluorescent properties when complexed with nucleic acids, and have utility in any application which requires detection of nucleic acids, such as detection of nucleic acids in solution, in gels, in blots, in microarrays, and in bacteria and cells, and for use in analysis of cell structure, membrane integrity, and function. The dyes of the invention have the formulawhere X is O, S, or C(CH3)2; Each of R1 and R6 is independently hydrogen, carboxy, sulfo, sulfonamide, halogen, CN, or alkoxy; Each of t and s is an integer from 0 to 4; R2 is an alkyl, or alkyl group substituted by a carboxy, or a sulfo; R3 is H, C6H4CO2R7, or C6H4CH2N(R7)2, where R7 is an alkyl; R4 is H, alkyl, aryl, halogen, alkoxy, alkylamino, or alkylthio; R5 is an alkyl, aryl, alkyl group substituted by a carboxy, or a sulfo, or —(CH2)a—[O—(CH2)b]m—O—Z, where Z is H, alkyl, or carboxy; each of a and b is an integer from 1 to 4; m is an integer selected from 0 to 4."},"analysis":{"summary":null,"layman_explanation":null,"technical_analysis":null,"business_analysis":null,"faqs":null,"topics":[],"tech_cluster":null},"seo":{"title":"Unsymmetrical cyanine dyes and their application","description":"The invention describes the preparation and use of fluorescent stains for nucleic acids derived from unsymmetrical cyanine dyes. The dyes of the invention possess superior fluorescent properties when ","keywords":[]},"attribution":{"source":"Patentable","source_url":"https://patentable.app","canonical_url":"https://patentable.app/patents/US-9850382","license":"CC-BY-4.0-like","license_terms":"AI-generated analysis on this page (summary, layman_explanation, technical_analysis, business_analysis, faqs) may be reused with attribution and a visible link back to the canonical URL above. Patent abstracts, claims, and bibliographic data are USPTO public domain.","required_link":"https://patentable.app/patents/US-9850382","citation_suggestion":"Patentable. \"Unsymmetrical cyanine dyes and their application\" (US-9850382). https://patentable.app/patents/US-9850382","copyright_holder":"Nomic Interactive Technology LLC"},"links":{"html":"https://patentable.app/patents/US-9850382","json":"https://patentable.app/api/llm-context/US-9850382","site":"https://patentable.app","llms_txt":"https://patentable.app/llms.txt"},"generated_at":"2026-06-06T11:18:50.275Z"}