{"schema_version":"1.0","canonical_url":"https://patentable.app/patents/US-9851309","patent":{"patent_number":"US-9851309","title":"Ultra-high-sensitive assay of protein and nucleic acid and kit, and novel enzyme substrate","assignee":null,"inventors":[],"filing_date":"2012-03-23T00:00:00.000Z","publication_date":"2017-12-26T00:00:00.000Z","cpc_codes":["G01N","C12Q","C12Q","C12Q","C12Q","G01N","G01N","G01N","G01N"],"num_claims":5,"abstract":"Provided is a ultra-high-sensitivity assay in which the assay can be made on a commonly used assay apparatus such as an absorptiometer and a plate reader or with naked eyes. The high-sensitivity assay in which the assay can be made on a commonly used assay apparatus or with naked eyes can be provided by combining an enzyme cycling method using thio-NAD(P) as a coenzyme, a labeling enzyme and a substrate for the labeling enzyme optimally, and by amplifying thio-NAD(P)H, which is a signaling substance, exponentially and then quantifying the thio-NAD(P)H colorimetrically."},"analysis":{"summary":null,"layman_explanation":null,"technical_analysis":null,"business_analysis":null,"faqs":null,"topics":[],"tech_cluster":null},"seo":{"title":"Ultra-high-sensitive assay of protein and nucleic acid and kit, and novel enzyme substrate","description":"Provided is a ultra-high-sensitivity assay in which the assay can be made on a commonly used assay apparatus such as an absorptiometer and a plate reader or with naked eyes. The high-sensitivity assay","keywords":[]},"attribution":{"source":"Patentable","source_url":"https://patentable.app","canonical_url":"https://patentable.app/patents/US-9851309","license":"CC-BY-4.0-like","license_terms":"AI-generated analysis on this page (summary, layman_explanation, technical_analysis, business_analysis, faqs) may be reused with attribution and a visible link back to the canonical URL above. Patent abstracts, claims, and bibliographic data are USPTO public domain.","required_link":"https://patentable.app/patents/US-9851309","citation_suggestion":"Patentable. \"Ultra-high-sensitive assay of protein and nucleic acid and kit, and novel enzyme substrate\" (US-9851309). https://patentable.app/patents/US-9851309","copyright_holder":"Nomic Interactive Technology LLC"},"links":{"html":"https://patentable.app/patents/US-9851309","json":"https://patentable.app/api/llm-context/US-9851309","site":"https://patentable.app","llms_txt":"https://patentable.app/llms.txt"},"generated_at":"2026-06-06T10:22:59.950Z"}