Legal claims defining the scope of protection, as filed with the USPTO.
1. A device for analyzing a fluorescence image comprising: a light source that emits light to a sample including a plurality of cells labeled with a fluorescent dye at a target site; an imaging unit including a camera that captures a fluorescence image of each of the cells that emit fluorescence by being irradiated with the light; and a processing device including a processor, a memory, a display, and an inputter, wherein the processor is configured to: process the captured fluorescence image to acquire a bright spot pattern of the fluorescence image; select a reference pattern corresponding to a measurement item of the sample from a plurality of reference patterns corresponding to a plurality of measurement items; compare the acquired bright spot pattern with the selected reference pattern; and generate information of the sample based on a comparison result of the acquired bright spot pattern and the selected reference pattern, wherein the measurement item detects a translocation of a target gene, a deletion of the target gene, an inversion of the target gene, or a duplication of the target gene.
2. The device according to claim 1 , wherein: the memory is configured to store the plurality of reference patterns corresponding to the plurality of measurement items, and the processor is configured to select the reference pattern corresponding to the measurement item of the sample from the stored plurality of reference patterns and generate the information used for determination of the sample.
3. The device according to claim 1 , wherein the processor is configured to generate, as the information used for determination of the sample, information of at least one of a number of abnormal cells, a ratio of the number of abnormal cells, a number of normal cells, and a ratio of the number of normal cells.
4. The device according to claim 1 , wherein the processor is configured to: determine, for each group of a plurality of cells included in the sample, whether or not the group of a plurality of cells is a group of abnormal cells by comparing the bright spot pattern of fluorescence in the fluorescence image with the reference pattern corresponding to the measurement item of the sample; and generate the information for determination of the sample based on a result of the determination made for each group of a plurality of cells.
5. The device according to claim 1 , wherein the measurement item is at least one selected from a group consisting of BCR/ABL fusion gene, AML1/ETO (MTG8) fusion gene, PML/BARα fusion gene, TEL/AML1 fusion gene, ALK gene, long arm deletion of chromosome 5, long arm deletion of chromosome 7, and long arm deletion of chromosome 20.
6. The device according to claim 2 , wherein the measurement item is further classified by probes to hybridize to the target site, and wherein the reference pattern is stored for each of the probes in the memory.
7. The device according to claim 6 , wherein the processor is configured to generate the information used for determination of the sample based on the bright spot pattern of fluorescence in the fluorescence image and the reference pattern selected from the plurality of reference patterns corresponding to the plurality of measurement items in correspondence with the probe of the measurement item of the sample.
8. The device according to claim 6 , wherein the measurement item is further classified into a typical positive pattern and a non-typical positive pattern for each of the probes to hybridize to the target site, and wherein the reference pattern is stored in the memory for each of the typical positive pattern and the non-typical positive pattern.
9. The device according to claim 1 , wherein the processor is configured to: select a fluorescence image of a cell determined to be an abnormal cell; and control the display to display the selected fluorescence image.
10. The device according to claim 9 , wherein the processor is configured to: correct a result of determination of an abnormal cell to a normal cell, wherein the abnormal cell is selected by the inputter from fluorescence images of abnormal cells displayed on the display.
11. The device according to claim 9 , wherein the processor is configured to: correct a result of determination of a normal cell to an abnormal cell, wherein the normal cell is selected by the inputter from fluorescence images of normal cells displayed on the display.
12. The device according to claim 2 , wherein the processor is configured to control the memory to store, as the reference pattern for each of the measurement items, a bright spot pattern of fluorescence in a fluorescence image of an abnormal cell that is newly acquired.
13. The device according to claim 1 , wherein the device further comprises: a pretreatment device that performs a pretreatment in which target sites in a plurality of cells in a sample are labeled with a fluorescent dye based on a set measurement item, wherein the processor is configured to: select the reference pattern corresponding to the measurement item of the sample from the plurality of reference patterns corresponding to the plurality of measurement items based on the measurement item set in the pretreatment device, and generate the information used for determination of the sample based on the bright spot pattern of fluorescence in the fluorescence image and the selected reference pattern.
14. The device according to claim 1 , further comprising: a flow cell through which the sample flows, wherein the light source irradiates the sample flowing through the flow cell with light.
15. An analyzing method comprising: identifying a measurement item of a sample including a cell; obtaining a fluorescence image of the cell that a target site is labeled with a fluorescence dye; selecting a reference pattern corresponding to the identified measurement item from a plurality of reference patterns corresponding to a plurality of measurement items, respectively; comparing a bright spot pattern of the fluorescence image with the selected reference pattern; and generating information of the sample based on a comparison result, wherein the measurement item detects a translocation of a target gene, a deletion of the target gene, an inversion of the target gene, or a duplication of the target gene.
16. The method according to claim 15 , wherein the reading of the reference pattern comprises: reading the reference pattern corresponding to the received measurement item of the sample from a plurality of reference patterns corresponding to the plurality of measurement items, wherein the plurality of reference patterns is stored in a memory.
17. The method according to claim 15 , wherein the generation of the information comprises: generating the information used for determination of the sample based on the bright spot pattern of fluorescence in the fluorescence image and the reference pattern selected from the plurality of reference patterns corresponding to the plurality of measurement items in correspondence with a probe of the measurement item of the sample.
18. The method according to claim 15 , further comprising: selecting a fluorescence image of a cell determined to be an abnormal cell; and displaying the selected fluorescence image on a display.
19. The method according to claim 18 , further comprising: correcting a result of determination of an abnormal cell to a normal cell, wherein the abnormal cell is selected by an inputter from fluorescence images of abnormal cells displayed on the display.
20. A non-transitory tangible media storing computer program for causing a computer to execute an analysis process of a fluorescence image of a cell obtained by imaging a sample including a plurality of cells in which a target site is labeled with a fluorescent dye, the computer program causing the computer to execute steps of: receiving a measurement item of the sample; reading a reference pattern corresponding to the received measurement item of the sample from a plurality of reference patterns corresponding to a plurality of measurement items; comparing a bright spot pattern of the fluorescence image with the read reference pattern; generating information of the sample based on a comparison result of the bright spot pattern and the read reference pattern; and displaying the generated information of the sample, wherein the measurement item detects a translocation of a target gene, a deletion of the target gene, an inversion of the target gene, or a duplication of the target gene.
21. The device according to claim 1 , wherein the processor is configured to: display the comparison result of the acquired bright spot pattern and the selected reference pattern.
22. The device according to claim 1 , wherein the reference pattern includes a positive pattern with which the processor identifies a positive cell.
23. The device according to claim 22 , wherein the positive pattern defines at least a number of fusion of bright spots, and the processor is configured to identify a positive cell based on the number defined by the positive pattern and a number of fusion of bright spots in the fluorescence image.
24. The device according to claim 1 , wherein the processor is configured to: select at least two reference patterns corresponding to a measurement item of the sample from the plurality of reference patterns corresponding to the plurality of measurement items.
25. The device according to claim 1 , wherein the processor is configured to: process the captured fluorescence image to acquire a bright spot pattern of a fluorescence image of a cell.
26. The method according to claim 15 , wherein the reference pattern includes a positive pattern having a positive cell.
27. The method according to claim 26 , wherein the positive cell has two fused bright spots including first bright spots and second bright spots overlapped in the fluorescence image.
28. The method according to claim 15 , wherein the reference pattern includes at least two reference patterns.
29. The method according to claim 15 , wherein the fluorescence image is an image of a cell.
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August 10, 2021
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