Optical Sectioning of a Sample and Detection of Particles in a Sample

1. A method for obtaining a plurality of images of a liquid sample, the method comprising: a) arranging said liquid sample in relation to a sample device; b) arranging said sample device in relation to an apparatus having: at least a first optical detection assembly comprising at least a first image acquisition device, said first optical detection assembly having an optical axis and an object plane, said object plane comprising an image acquisition area from which electromagnetic waves can be detected as an image by said first image acquisition device; an image illumination device; at least one translation unit configured to move said sample device and said first optical detection assembly relative to each other; and said apparatus arranged to support said first optical detection assembly and said translation unit, wherein said first optical detection assembly and said translation unit are configured so that at least a part of said sample device is intersected by said image acquisition area; c) moving said sample device and said first detection assembly relative to each other over a scanning length, said movement comprising a movement in a direction along a first scanning path, which defines an angle theta relative to said optical axis, wherein theta is in the range of from about 0.3 to about 89.7 degrees, wherein said at least one translation unit moves said sample device and said at least one optical detection assembly relative to each other in a substantially continuous step-wise manner; d) wherein electromagnetic waves are transmitted from the image illumination device through the image acquisition area towards the image acquisition device; and e) obtaining said plurality of images, wherein the plurality of images are obtained with a predetermined time interval in between two successive images, wherein the predetermined time interval is in the range of from about 10 −9 s to about 10 3 s.

2. The method according to claim 1 , further comprising analyzing said images recorded by said first image acquisition device, comprising identifying edges of objects in said images, wherein at least one pattern recognition algorithm is used to analyze said images for distinguishing between regions of different brightness in said images or for identifying transitions between brighter and darker regions in said image.

3. The method according to claim 2 , wherein a number of images depicting adjacent parts of a given sample are analyzed to determine at which position of said object plane along said scanning path an object appearing in several of said images is in focus.

4. The method according to claim 3 , comprising determining the area of said object in said images.

5. The method according to claim 4 , wherein the position of said object plane along said scanning path at which said object is in focus is determined from a curve depicting the area of said object in said images versus the position along said scanning path.

6. The method according to claim 5 , wherein at least one sample boundary of said sample to its surroundings is identified in at least one of said images.

7. The method according to claim 1 , wherein when said sample is arranged in relation to the sample device comprising a support, said support is substantially parallel to said scanning path.

8. The method according to claim 7 , wherein said sample is a drop of a liquid sample arranged on said support, said method comprising identifying a first sample boundary comprising an interface between said sample and said support, and identifying a second sample boundary comprising an interface between said sample and the surrounding atmosphere.

9. The method according to claim 8 , comprising determining the separation of said first and second boundaries.

10. The method according to claim 9 , comprising determining the analyzed volume of said sample from said separation of said first and second sample boundaries, the scanned length of said sample and the dimension of said image acquisition area.

11. The method according to claim 10 , comprising determining the analyzed part of said volume of said sample from the separation of said first and second sample boundaries, the separation of a third and fourth sample boundaries, and the scanned length of said sample.

12. The method according to claim 1 , further comprising reading information provided by a coding on said sample device, such as information relating to the position on the sample device, allowing a determination of the location along said scanning length where each of said plurality of images are acquired.

13. The method according to claim 1 , comprising obtaining and analyzing images until a predetermined number of particles with a predetermined quality have been identified, and wherein the size of the imaged and analyzed part of said volume of said inhomogeneous liquid sample is determined.

14. The method according to claim 13 , comprising determining the concentration of said particles with a predetermined quality in said inhomogeneous liquid sample from said predetermined number of particles with a predetermined quality and said size of the imaged and analyzed part of said volume.

15. The method according to claim 1 , comprising obtaining said plurality of images by scanning said sample once along said scanning path.

16. The method according to claim 1 , wherein said analysis combines a plurality of scans along said scanning path, each scan comprising at least one image.

17. The method according to claim 16 , where at least one scan is made with a first step length and a first image resolution, where at least a second scan is made with a second step length and a second image resolution, and wherein said first step length is larger than or equal to said second step length, and wherein said first image resolution is lower than or equal to said second image resolution.

18. The method according to claim 1 , comprising determining at least one parameter of a volume of an inhomogeneous liquid sample arranged in relation to said sample device.

19. The method according to claim 1 , wherein said translation unit moves said sample device and said at least one optical detection assembly relative to each other in substantially identical steps of a predetermined step length in between two successive images, wherein the predetermined step length is in the range of from about 0.05 micrometers to 1000 micrometers.

20. The method according to claim 1 , further comprising moving said sample device and said first optical detection assembly relative to each other along an axis having an angle to said scanning path.

21. The method according to claim 1 , said first scanning path being selected from the group consisting of a scanning axis or a tangential to a rotational movement.

22. The method according to claim 1 , where the sample is an inhomogeneous liquid sample.

23. A method for obtaining a plurality of images of an inhomogeneous liquid sample, said method comprising: a) arranging said inhomogeneous liquid sample in relation to a sample device; b) arranging said sample device in relation to an apparatus having: at least a first optical detection assembly comprising at least a first image acquisition device, said first optical detection assembly having an optical axis and an object plane, said object plane comprising an image acquisition area from which electromagnetic waves can be detected as an image by said first image acquisition device; an image illumination device; at least one translation unit configured to move said sample device and said first optical detection assembly relative to each other; and said apparatus arranged to support said first optical detection assembly and said translation unit, wherein said first optical detection assembly and said translation unit are configured so that at least a part of said sample device is intersected by said image acquisition area; c) moving said sample device and said first detection assembly relative to each other over a scanning length, said movement comprising a movement in a direction along a first scanning path, which defines an angle theta relative to said optical axis, wherein theta is in the range of from about 0.3 to about 89.7 degrees, and wherein said at least one translation unit moves said sample device and said at least one optical detection assembly relative to each other in a substantially continuous step-wise manner; d) wherein electromagnetic waves are transmitted from the image illumination device through the image acquisition area towards the image acquisition device; and e) obtaining said plurality of images, wherein the plurality of images are obtained with a predetermined time interval in between two successive images, wherein the predetermined time interval is in the range of from about 10 −9 s to about 10 3 s.