Methods and systems for laser scan location verification and laser surgical systems with laser scan location verification

A method of verifying a laser scan at a predetermined location within an object includes imaging at least a portion of the object, the resulting image comprising the predetermined location; identifying the predetermined location in the image, thereby establishing an expected scan location of the laser scan in the image; performing a laser scan on the object by scanning a focal point of the laser beam in a scanned area; detecting a luminescence from the scanned area and identifying an actual scanned location within the image based on the detected luminescence; and determining whether the difference between the actual scanned location and the expected scan location is within a threshold value.