Inverted light-sheet microscope

Devices and techniques for a light sheet microscope device are generally described. In some examples, the light sheet microscope may comprise a motorized movable stage comprising an optically clear glass plate. In some further examples, the light sheet microscope may comprise an illumination objective and a collection objective disposed on a first side of the optically clear glass plate. In some further examples, the light sheet microscope may comprise a wavefront- and index-matching element disposed on the first side of the optically clear glass plate. An oil layer may be disposed between the optically clear glass plate and the wavefront- and index-matching element. The oil layer, the wavefront- and index-matching element and the glass plate may have matching refractive indexes.