Image acquisition device and image acquisition method

An image acquisition apparatus includes a spatial light modulator, an optical scanner, a detection unit, a control unit. The spatial light modulator performs focused irradiation on irradiation regions on a surface or inside of an observation object with modulated excitation light. The detection unit has imaging regions in an imaging relation with the irradiation regions on a light receiving surface, each of the imaging regions corresponds to one or two or more pixels, and a pixel that corresponds to none of the imaging regions exists adjacent to each imaging region. The control unit corrects a detection signal of a pixel corresponding to each imaging region on the basis of a detection signal of the pixel that exists adjacent to the imaging region and corresponds to none of the imaging regions, and generates an image of the observation object on the basis of the corrected detection signal.