Disclosed is an apparatus for enriching and screening a target object such as a cell, bacteria or biomolecule from a sample. The apparatus includes a plurality of multi-channel mesh bodies arranged in parallel, and each multi-channel mesh body includes a mesh body formed by intersecting a plurality of threads, a plurality of screen pores formed by intersecting the plurality of threads, and a capturing layer formed on the mesh body. The capturing layer includes a capturing material specifically binding with the target object.
Legal claims defining the scope of protection, as filed with the USPTO.
1. An enriching and screening apparatus, comprising: a main body comprising an inlet, an outlet, and a cavity located between the inlet and the outlet; and a plurality of multi-channel mesh bodies fixed within the cavity, each multi-channel mesh body of the plurality of multi-channel mesh bodies comprising: a mesh body formed by intersecting mesh wire, the intersecting mesh wire including a first plurality of mesh wires extending in a first direction that intersect with a second plurality of mesh wires extending in a second direction that is transverse to the first direction; a plurality of screen pores, the plurality of screen pores defined by openings located between the first plurality of mesh wires and the second plurality of mesh wires; and a capturing layer bonded to the mesh body, the capturing layer including capturing materials configured to bind with a target object, wherein the capturing materials are selected from antibodies, phages, or aptamers; wherein the plurality of multi-channel mesh bodies are arranged in a vertical direction within the cavity such that at least a first multi-channel mesh body of the plurality of multi-channel mesh bodies is positioned in a first horizontal plane and a second multi-channel mesh body of the plurality of multi-channel mesh bodies is positioned in a second horizontal plane that is below the first horizontal plane, and a space between the first horizontal plane and the second horizontal plane is 0.1-10 mm; wherein screen pores of the first multi-channel mesh body are at least partially staggered with respect to screen pores of the second multi-channel mesh body when the plurality of multi-channel mesh bodies are arranged in the vertical direction within the cavity; wherein the screen pores of the first multi-channel mesh body are at least partially staggered with respect to the screen pores of the second multi-channel mesh body as a function of the first multi-channel mesh body being oriented at an angle with respect to the second multi-channel mesh body, and the angle is between 30-60°; wherein a thickness of the intersecting mesh wire ranges from 10 μm to 500 μm; wherein capturing materials of a capturing layer of the first multi-channel mesh body are the same as capturing materials of a capturing layer of the second multi-channel mesh body.
2. The enriching and screening apparatus according to claim 1, wherein the thickness ranges from 20 μm to 200 μm.
3. The enriching and screening apparatus according to claim 1, wherein the openings located between the first plurality of mesh wires and the second plurality of mesh wires have from 700 to 2000 μm2.
4. The enriching and screening apparatus according to claim 1, wherein a number of the plurality of multi-channel mesh bodies ranges from 2 to 30.
5. The enriching and screening apparatus according to claim 1, wherein a number of the plurality of multi-channel mesh bodies ranges from 3 to 15.
6. The enriching and screening apparatus according to claim 1, wherein a cross-section of the openings is square, rectangular, triangular, polygonal, circular, parallelogram, or trapezoid.
Cooperative Patent Classification codes for this invention. Click any code to explore related patents in that topic.
October 10, 2018
March 25, 2025
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