A sample separation device includes a separation path and a control unit. The separation path is between a fluid drive and a sample separation unit, and is configured to inject a fluidic sample in an injector path into a mobile phase in the separation path driven by the fluid drive to the sample separation unit for chromatographically separating the fluidic sample. The control unit is configured to control blocking the injector path or at least a partial path thereof, generating an overpressure in the blocked injector path or the partial path thereof by a pressure source, and subsequently fluidically coupling the injector path or the partial path thereof to a region of lower pressure than the overpressure to generate an expansion stroke for releasing unwanted components from the injector path.
Legal claims defining the scope of protection, as filed with the USPTO.
1. A separation device, comprising: a separation path between a fluid drive and a sample separation unit, wherein the sample separation device is configured to inject a fluidic sample in an injector path into a mobile phase in the separation path driven by the fluid drive to the sample separation unit for chromatographically separating the fluidic sample; and a control unit configured to control an operation comprising: blocking the injector path or at least a partial path thereof; generating an overpressure in the blocked injector path or the partial path thereof by a pressure source; and subsequently fluidically coupling the injector path or the partial path thereof to a region of lower pressure than the overpressure to generate an expansion stroke for releasing unwanted components from the injector path.
2. The sample separation device of claim 1, wherein the fluid drive is configured to generate a system pressure in the separation path for driving the mobile phase and the fluidic sample to the separation unit, and the control unit is configured to generate the overpressure at a pressure corresponding to the system pressure.
3. The sample separation device of claim 2, wherein the system pressure is selected from the group consisting of: a pressure of at least 500 bar; a pressure of at least 1000 bar; and a pressure of at least 1200 bar.
4. The sample separation device of claim 1, wherein the control unit is configured to generate the overpressure at a pressure selected from the group consisting of: a pressure in a range from 400 bar to 2000 bar; and a pressure in a range from 700 bar to 1500 bar.
5. The sample separation device of claim 1, wherein the region of lower pressure comprises a waste conduit.
6. The sample separation device of claim 1, wherein the region of lower pressure is at ambient pressure.
7. The sample separation device of claim 1, wherein, simultaneously with and/or after generating the expansion stroke, the control unit is configured to control rinsing the injector path to discharge the unwanted components to the region of lower pressure.
8. The sample separation device of claim 1, wherein the pressure source is selected from the group consisting of: a metering unit for metering the fluidic sample in the injector path; a rinsing pump for rinsing the injector path; the fluid drive; and a pump that is separate from the fluid drive.
9. The sample separation device of claim 1, wherein the unwanted components comprise at least one of: one or more particles; one or more sample residues; one or more bubbles.
10. The sample separation device of claim 1, comprising an injection valve controllable by the control unit to block the injector path or the partial path thereof and to subsequently fluidically couple the injector path or the partial path thereof to the region of lower pressure.
11. The sample separation device of claim 1, wherein the control unit is configured to control injecting the fluidic sample in the injector path into the mobile phase in the separation path by flow-through injection, by which a sample reception volume containing the fluidic sample is connected into the separation path between the fluid drive and the sample separation unit.
12. The sample separation device of claim 1, wherein the control unit is configured to control injecting the fluidic sample in the injector path into the mobile phase in the separation path by feed injection, by which the injector path is connected to the separation path at a fluidic T-point to combine the fluidic sample with the mobile phase.
13. A method for operating a sample separation device, the method comprising: providing a separation path between a fluid drive and a sample separation unit of the sample separation device, wherein the sample separation device is configured to inject a fluidic sample in an injector path into a mobile phase in the separation path driven by the fluid drive to the sample separation unit for chromatographically separating the fluidic sample; blocking the injector path or at least a partial path thereof; generating an overpressure in the blocked injector path or the partial path thereof by a pressure source; and subsequently fluidically coupling the injector path or the partial path thereof to a region of lower pressure than the overpressure to generate an expansion stroke for releasing unwanted components from the injector path.
14. The method of claim 13, wherein the fluid drive is configured to generate a system pressure in the separation path for driving the mobile phase and the fluidic sample to the separation unit, and the method comprises generating the overpressure at a pressure corresponding to the system pressure.
15. The method of claim 14, wherein the system pressure is selected from the group consisting of: a pressure of at least 500 bar; a pressure of at least 1000 bar; and a pressure of at least 1200 bar.
16. The method of claim 13, wherein the overpressure is generated at a pressure selected from the group consisting of: a pressure in a range from 400 bar to 2000 bar; and a pressure in a range from 700 bar to 1500 bar.
17. The method of claim 13, wherein the region of lower pressure comprises a waste conduit.
18. The method of claim 13, wherein the region of lower pressure is at ambient pressure.
19. The method of claim 13, comprising, simultaneously with and/or after generating the expansion stroke, rinsing the injector path to discharge the unwanted components to the region of lower pressure.
20. The method of claim 13, wherein the unwanted components comprise at least one of: one or more particles; one or more sample residues; one or more bubbles.
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February 1, 2024
May 27, 2025
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