A synthetic strategy for the creation of large scale chemical diversity. Solid-phase chemistry, photolabile protecting groups, and photolithography are used to achieve light-directed spatially-addressable parallel chemical synthesis. Binary masking techniques are utilized in one embodiment. A reactor system, photoremovable protective groups, and improved data collection and handling techniques are also disclosed. A technique for screening linker molecules is also provided.
Legal claims defining the scope of protection, as filed with the USPTO.
1. An array of polymers, the array comprising: a planar solid support; and a plurality of different polymers attached to a surface of the planar solid support at a density exceeding 400 different polymers/cm 2 , wherein each of the different polymers is attached to the surface of the planar solid support in a different known location and, it is known which polymer occupies which known location.
2. The array of claim 1 , wherein the polymers are peptides.
3. The array of claim 2 , wherein each different polymer is from 4 to 20 monomers in length.
4. The array of claim 2 , wherein each different polymer is at least 10 monomers in length.
5. The array of claim 2 , wherein each different polymer is at least 20 monomers in length.
6. The array of claim 1 , wherein the array comprises at least 1,000 different polymers attached to the surface of the planar solid support.
7. The array of claim 1 , wherein the array comprises at least 10,000 different polymers attached to the surface of the planar solid support.
8. The array of claim 1 , wherein each of the different known locations is physically separated from each other of the different known locations.
9. The array of claim 1 , wherein said planar solid support is glass.
10. The array of claim 1 , wherein said polymers are attached to the surface of the planar solid support through a linker group.
11. The array of claim 1 , wherein the polymers in the different known locations are at least 20% pure.
12. The array of claim 1 , wherein the polymers in the different known locations are at least 50% pure.
13. The array of claim 1 , wherein the polymers in the different known locations are at least 80% pure.
14. The array of claim 1 , wherein the polymers in the different known locations are at least 90% pure.
15. The array of claim 1 wherein the different known locations are square or rectangular in shape.
16. The array of claim 1 , wherein the substrate is rigid.
17. The array of claim 1 , wherein the array is produced by a binary synthesis process.
18. The array of any of claim 1 , wherein the substrate is glass.
19. The array of claim 1 , wherein the polymers are nucleic acids.
Cooperative Patent Classification codes for this invention. Click any code to explore related patents in that topic.
November 17, 1999
February 12, 2002
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