Disclosed is a rapid method for the separation of small organic compounds using gradient reverse phase HPLC. The method achieves a run time of one minute or less and a resolution with a peak production of at least 1 peak/2 seconds. The method is also able to achieve the separation of a compound from a mixture of compounds in an elution sample having a volume of 2 milliliters of less.
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September 12, 2000
December 24, 2002
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