A novel method for determining the bioactivity of TGF-β in a sample of milk, raw protein source, or nutritional composition is provided. The method includes particular reconstitution steps, centrifugation steps, incubation steps, and activation steps. The bioactivity of the TGF-β in the sample may be measured in a HT-2 cell bioassay or a cellomics bioassay.
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February 13, 2009
January 8, 2013
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